Composite
Part:BBa_K5403021:Design
Designed by: Lizette van der Ziel Group: iGEM24_TU-Eindhoven (2024-10-02)
GFP with N-terminal Spytag and C-terminal hexahistidine tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 481
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
GFP and Spytag are joined via a flexible linker (BBa_K5403002), to avoid that the conjugation of these two would cause misfolding.
Source
S. pyogenes (spytag) and Aequoria victoria (GFP).