Composite

Part:BBa_K5403021:Design

Designed by: Lizette van der Ziel   Group: iGEM24_TU-Eindhoven   (2024-10-02)


GFP with N-terminal Spytag and C-terminal hexahistidine tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 481
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GFP and Spytag are joined via a flexible linker (BBa_K5403002), to avoid that the conjugation of these two would cause misfolding.


Source

S. pyogenes (spytag) and Aequoria victoria (GFP).

References